Degree and area of specialization: Bachelor's Degree in molecular biology/ biological sciences is required.
Minimum number of years and type of relevant work experience:
Minimum work experience of at least 1-2 years of previous laboratory experience and experience with experimental cerebral ischemia surgery in rats/mice is required.
Principal duties: The research specialist will perform research related to understanding the role of the innate immune system in repair and recovery following focal cerebral ischemia.
50% experimental ischemia surgeries in rats/mice
Performing Middle Cerebral Artery Occlusion surgeries in rats and mice
Using Laser Doppler to check cerebral blood flow during above surgeries
Post-op care of MCAO animals
Neurobehavioral assessment of animals post-op
Dissection, collection and preparation of brains after sacrifice - Sacrifice and perfusion of above animals after specific time points
15% Molecular biology, Histology and Microscopy, tissue culture techniques
RT-PCR, qPCR, RNA sequencing and Western blots
Analysis of gene expression in rodent and human brain tissues
Microscopy: bright field and fluorescent techniques, 3-D imaging
Histology: Preparation of rodent brains for cryosectioning and immunofluorescence histochemistry
Maintain cell lines in culture.
10% General lab duties:
Purchasing and managing inventory of laboratory supplies and general lab maintenance.
Performing required recordkeeping such as hazardous waste disposal
Training and working with students and undergraduate helpers.
Resolve unexpected/unplanned issues as they arise.
15% Assist and teach students lab techniques and procedures and help others with their experiments
10% Semi-independent research project responsibilities
POSITION VACANCY LISTING University of Wisconsin-Madison
Additional Information: Good written and oral communication skills and ability to perform literature searches. A 12 month evaluation period will be required.
Position Summary: The candidate will perform experimental focal ischemia surgery in rodents and perform other biological/molecular biology techniques such as Western blotting, tissue culture, PCR, etc., as well as lab managerial duties.
The School of Medicine and Public Health has a deep and profound commitment to diversity both as an end in itself but also as a valuable means for eliminating health disparities. As such, we strongly encourage applications from candidates who foster and promote the values of diversity and inclusion.
A period of evaluation will be required.
Internal Number: 021319
About The University of Wisconsin
The University of Wisconsin is one of the top 10 NIH-funded public universities. It is located in Madison, a beautiful, multicultural, capital city situated on an isthmus between lake Monona and lake Mendota.